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Serodiagnosis of tuberculous lymphadenitis using a combination of antigens


Document type: article
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Abstract: BACKGROUND: The diagnosis of extra-pulmonary tuberculosis (EPTB) by conventional methods such as culture and microscopy has low sensitivity and requires an invasive procedure. A simple rapid serological test would be of great value. METHODOLOGY: Six antigens (ESAT-6, Ag85A, TB10.4, Rv3881c, lipoarabinomannan (LAM) and Ara6-BSA) were tested in an ELISA to detect antigen-specific IgG and IgM antibodies in sera from 54 culture- and histology-confirmed tuberculous lymphadenitis (TBLN) patients as follows: four were HIV seropositive; sera from 25 was smear positive for pulmonary tuberculosis (PTB); 15 were culture- and histology-negative lymphadenitis (non-TBLN) patients; and 22 werehealthy controls (HCs). RESULTS: The sensitivities of the antigens for the detection of IgG in sera of TBLN patients ranged from 4% to 30%. Specificities ranged from 73% to 100% with sera from non-TBLN patients and 91% to 100% with sera from HCs. Sensitivities of the antigens for detection of IgM ranged from 0% to 15% and specificities ranged from 80% to 100% with sera from non-TBLN patients and 91% to 100% with sera from HCs. LAM was the most potent antigen for detection of IgG. When LAM and ESAT-6 were combined, sensitivity increased up to 43% and specificity with non-TBLN was 80% with HC 96%. CONCLUSIONS: The study suggests that the combined use of LAM and ESAT-6 for IgG antibody detection in sera from TBLN patients could be a supplement to microscopy of fine-needle aspirate (FNA) to diagnose TBLN among patients suspected of TBLN.
Authors: Beyene, D. , Franken, K.L. , Yamuah, L. , Aseffa, A. , Wiker, H.G. , Kolk, A. , Engers, H. , Klatser, P. , Sviland, L.
Category: Research
End Page: 102
Serial number: 2
ISSN: [1972-2680]
Volume: 4
Journal: Journal of Infection in Developing Countries
Keywords: health , disease prevention and control
Language: eng
Organization: KIT - Royal Tropical Institute
Year: 2010
Right: © 2010 Beyene et al. This article is licensed under a Creative Commons Attribution License.
Subject: Health and Nutrition
Start Page: 96
Title: Serodiagnosis of tuberculous lymphadenitis using a combination of antigens