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Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis


Document type: article
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Abstract: BACKGROUND: Molecular methods to detect Leishmania parasites are considered specific and sensitive, but often not applied in endemic areas of developing countries due to technical complexity. In the present study isothermal, nucleic acid sequence based amplification (NASBA) was coupled to oligochromatography (OC) to develop a simplified detection method for the diagnosis of leishmaniasis. NASBA-OC, detecting Leishmania RNA, was evaluated using clinical samples from visceral leishmaniasis patients from East Africa (n = 30) and cutaneous leishmaniasis from South America (n = 70) and appropriate control samples. RESULTS: Analytical sensitivity was 10 parasites/ml of spiked blood, and 1 parasite/ml of culture. Diagnostic sensitivity of NASBA-OC was 93.3% (95% CI: 76.5%-98.8%) and specificity was 100% (95% CI: 91.1%-100%) on blood samples, while sensitivity and specificity on skin biopsy samples was 98.6% (95% CI: 91.2%-99.9%) and 100% (95% CI: 46.3%-100%), respectively. CONCLUSION: The NASBA-OC format brings implementation of molecular diagnosis of leishmaniasis in resource poor countries one step closer.
Authors: Mugasa, C.M. , Laurent, T. , Schoone, G.J. , Basiye, F.L. , Saad, A.A. , Safi, S. el , Kager, P.A. , Schallig, H.D.F.H.
Country: Sudan , Suriname , Brazil
Category: Research
Serial number: 13
ISSN: [1756-3305]
Volume: 3
Journal: Parasites & vectors
Keywords: health , disease prevention and control
Language: eng
Organization: KIT - Royal Tropical Institute
PAGE: 6
Year: 2010
Region: East Africa , South America
Right: © 2010 Mugasa et al. This work is licensed under a Creative Commons Attribution License
Subject: Health and Nutrition
Title: Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis