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Titers rise over some weeks and commonly are detectable for life. Cell cultures for the plaque reduction neutralization test (PRNT) can be used aa are complement fixation (CFF) tests. Hemagglutination inhibition (HI) titers persist for several weeks to decline yet remain detectable for life. Dengue type-specific enzyme-linked immunosorbent assay (ELISA) can determine whether an infection is recent from one sample. The advent of the polymerase chain reaction has radically reduced the time needed for diagnosis.