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Then the other materials are tested and their LC50 and LT50 values are plotted as shown.

In this protocol, the same level of effect must be achieved, i.e. 50% lethality (LC50) and we ask what will be the concentration to kill 50% (LC50) and the time to do this (LT50).

Once the results are obtained a variety of comparisons can be made from the plotted data as shown on the next page.